Expanding the application of lignin peroxidases from <i>Irpex Lacteus</i> in Lignin degradation by exploring the effects of small molecules on enzyme activity

Lignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of for environmental prevention and governance was promoted by exploring effects small molecule substances on activities. In this study, (LiP) gene from Irpex Lacteus heterologous expressed Escherichia coli. The activity recombinant LiP 11.2 U/mg, optimum temperature 37℃, pH 3.5. range 20-45℃ 2.0-4.0, relative enzyme remained above 50%, showing good acid resistance, Zn 2+ , malonic acid, glycerol other promote enzyme, among which increase to 256%, when supplemental level is 3%, 186%. This study first time verified promoting effect activity, serving as reference studies breakdown paper wastewater treatment.